Note: If you see two issues marked, that denotes a difference in cameo and full appearances.
Marvel Heroes First Appearances #
Abigail Brand (Astonishing X-Men #3/#6, 2004)
Agatha Harkness (Fantastic Four vol. 1 #94, 1970)
Agent Venom (Secret Avengers #23, 2012)
Alpha Flight (X-Men #120, 1979)
America Chavez (Vengeance #1, 2011)
Angel (X-Men #1, 1963)
Angela (Odinsdottir) (Age of Ultron #10, 2013)
Anti-Venom (Amazing Spider-Man vol. 1 #569, 2008)
Ant Man (Tales to Astonish #27, 1962)
Archangel (X-Factor #24, 1988)
Avengers, The (Avengers #1, 1963)
Azari T’Challa (Avengers vol. 4 #1/#6, 2010)
Beast (X-Men #1, 1963)
Ben Reilly (Amazing Spider-Man vol. 1 #149, 1975)
Benji Parker (Spider-Girl #59, 2003)
Beta Ray Bill (Thor #337, 1983)
Big Hero 6 (Sunfire & Big Hero 6 #1, 1998)
Bishop (Uncanny X-Men #282, 1991)
Black Bolt (Fantastic Four #45, 1965)
Black Knight (Avengers #48, 1968)
Black Panther (Fantastic Four #52, 1966)
Black Panther II (Shuri) (Black Panther vol. 5 #5, 2009)
Black Widow (Tales of Suspense #52, 1964)
Blade (Tomb of Dracula #10, 1973)
Blind Al (Althea) (Deadpool vol. 3 #1 1997)
Bob, Agent of Hydra (Cable & Deadpool #38, 2007)
Boom Boom (Tabitha Smith) (Secret Wars II #5, 1985)
Brother Voodoo (Strange Tales #169, 1973)
Cable (New Mutants #87, 1990)
Captain America (Captain America Comics #1, 1941)
Captain America VII (James Buchanan Barnes) (Captain America vol. 5 #34, 2008)
Captain America VIII (Sam Wilson) (Captain America vol. 7 #25, 2014)
Captain America (Peggy Carter) (Exiles vol. 3 #3 2018)
Captain Marvel (Mar-Vel) (Marvel Super-Heroes #12, 1967)
Captain Marvel (Monica Rambeau) (Amazing Spider-Man Annual #16, 1982)
Captain Marvel (Carol Danvers) (Astonishing Spider-Man #9, 2012)
Captain Universe (Micronauts #8, 1979)
Carol Danvers (Marvel Super-Heroes #13, 1968)
Cassie Lang (Marvel Premiere #47 1979)
Cat, The (The Cat #1, 1972)
Clea (Strange Tales #126, 1964)
Cloak & Dagger (Spectacular Spider-Man #64 1982)
Colossus (Giant-Size X-Men #1, 1975)
Conan (Conan the Barbarian #1, 1970)
Cosmic Ghost Rider (Frank Castle) (Thanos vol. 2 #13, 2018)
Cyclops (X-Men #1, 1963)
Danger (Astonishing X-Men #9, 2005)
Daredevil (Daredevil #1, 1964)
Dazzler (Uncanny X-Men #130)
Deadpool (New Mutants #98, 1991)
Deathlok (Astonishing Tales #25, 1974)
Defenders (Marvel Feature #1, 1971)
Devil Dinosaur (Devil Dinosaur #1, 1978)
Doc Samson (Incredible Hulk #141, 1971)
Doctor Strange (Strange Tales 110, 1963)
Domino (Neena Thurman) (X-Force #11, 1992)
Dora Milaje (Black Panther vol. 3 #1, 1998)
Drax (Iron Man #55, 1973)
Dylan Brock (Venom vol. 4 #9, 2019)
Echo (Maya Lopez) (Daredevil vol. 2 #9, 1999)
Elektra (Daredevil #168, 1981)
El Muerto (Juan-Carlos Sanchez) (Friendly Neighborhood Spider-Man #6, 2006)
Ezekiel (Ezekiel Sims) (Amazing Spider-Man vol. 2 #30, 2001)
Falcon, The (Captain America #117, 1969)
Fantastic Four (Fantastic Four #1, 1961)
Fantomex (New X-Men #128, 2002)
Forge (Uncanny X-Men #184, 1984)
Gambit (Uncanny X-Men #266, 1990)
Gamora (Strange Tales #180, 1975)
Ghost Rider (Carter Slade) (The Ghost Rider #1, 1967)
Ghost Rider (Danny Ketch) (Ghost Rider vol. 3 #1, 1990)
Ghost Rider (Johnny Blaze) (Marvel Spotlight #5, 1972)
Ghost Rider (Rex Fury) (Tim Holt #6, 1949)
Ghost Rider (Robbie Reyes) (All-New Ghost Rider #1, 2014)
Giant Man (Tales to Astonish #49, 1963)
Gorgon (Fantastic Four #44, 1965)
Grey Hulk (Incredible Hulk #324, 1986)
Groot (Tales to Astonish #13, 1960)
Guardians of the Galaxy (Marvel Super-Heroes #18, 1969)
Gwen Stacy (Amazing Spider-Man #31, 1965)
Gwenom (Spider-Gwen #24, 2017)
Happy Hogan (Tales of Suspense #45, 1963)
Harry Osborn (Amazing Spider-Man #31, 1965)
Havok (X-Men #58, 1969)
Hawkeye (Tales of Suspense #57, 1964)
Hellcat (Avengers #144, 1976)
Henry Pym (Tales to Astonish #27, 1962)
Her (Ayesha) (Fantastic Four vol. 3 #11, 1998)
Hercules (Journey Into Mystery Annual #1, 1965)
HIM (Warlock) (Fantastic Four #67, 1967)
Hit Monkey (Hit Monkey #1, 2010)
Honey Badger (Gabrielle “Gabby” Kinney) (All-New Wolverine #2, 2016)
Hope Pym (A-Next #7, 1999)
Hope Summers (X-Men vol. 2 #205, 2008)
Howard the Duck (Adventures Into Fear #19, 1973)
Hulk (Incredible Hulk #1, 1962)
Ice Man (X-Men #1, 1963)
Illuminati (New Avengers #7, 2005)
Immortal Hulk (Avengers vol. 6 #684, 2018)
Inhumans (Fantastic Four #45, 1965)
Iron Man (Tales of Suspense #39, 1963)
Invaders, The (Avengers #71, 1969)
Iron Fist (Marvel Premier #15, 1974)
Iron Spider (Amazing Spider-Man vol. 1 #529, 2006)
Jessica Jones (Alias #1, 2001)
James Rhodes (Iron Man #118, 1979)
John Walker (Captain America vol. 1 #323, 1986)
Jubilee (Uncanny X-Men #244, 1989)
Kamala Khan (Captain Marvel vol. 6 #14, 2013)
Kid Loki (Loki Laufeyson) (Might Thor #617, 2011)
Kid Omega (Quentin Quire) (New X-Men #134, 2003)
Kitty Pryde (X-Men #129, 1980)
Lady Deadpool (Wanda Wilson) (Deadpool: Merc with a Mouth #7, 2010)
Legion (New Mutants #25/#26, 1985)
Luke Cage (Hero for Hire #1, 1972)
Madame Web (Amazing Spider-Man vol. 1 #210, 1980)
Mania (Andrea Benton) (Venom vol. 2 #38, 2013)
Mantis (Avengers #112, 1973)
Man-Wolf (Amazing Spider-Man #124, 1973)
Mary Jane (Amazing Spider-Man #42, 1966)
Medusa (Fantastic Four #36, 1965)
Midnight Sons (Ghost Rider vol. 3 #28, 1992)
Miles Morales (Ultimate Fallout #44, 2011)
Ms. Marvel (Carol Danvers) (Ms. Marvel #1, 1977)
Ms. Marvel (Kamala Khan) (Ms. Marvel #1, 2014)
Mobius M. Mobius (Fantastic Four vol. 1 #353, 1991)
Mockingbird (Marvel Team-Up #95, 1980)
Moon-Boy (Devil Dinosaur #1, 1978)
Moon Knight (Werewolf by Night #32, 1975)
Molecule Man (Fantastic Four #20, 1963)
Nick Fury (Sgt. Fury and his Howling Commandos #1, 1963)
Nick Fury Jr. (Marcus Johnson) (Battle Scars #1, 2012)
Nightcrawler (Giant-Size X-Men #1, 1975)
Nova (Richard Rider) (Nova #1, 1976)
Nova II (Sam Alexander) (Point One #1 2012)
Odin (Journey Into Mystery #85, 1962)
Old Man Logan (Wolverine vol. 3 #66, 2008)
Pepper Potts (Tales of Suspense #45, 1963)
Pete Wisdom (Excalibur vol.1 #86, 1995)
Phil Coulson (Battle Scars #6, 2012)
Professor X (X-Men #1, 1963)
Pip the troll (Warlock #12, 1976)
Psylocke (New Mutants Annual #2, 1986)
Punisher, The (Amazing Spider-Man #129, 1974)
Phyla-Vell (Captain Marvel vol. 4 #16/#17, 2003)
Quasar (Captain America #217, 1978)
Quicksilver (X-Men 4, 1964)
Red Hulk (Hulk vol. 3 #1, 2008)
Red She-Hulk (Betty Ross) (Hulk vol. 3 #15/#16, 2009)
Red Sonja (Conan: The Barbarian #23/#24, 1973)
Red Wolf (Avengers #80, 1970)
Riri Williams (Invincible Iron Man vol. 3 #9, 2016)
Rocket Raccoon (Marvel Preview #7, 1976)
ROM (ROM Spaceknight #1, 1979)
Rogue (Avengers Annual #10, 1981)
Runaways (Runaways #1, 2003)
Scarlet Spider (Web of Spider-Man #118, 1994)
Scarlet Witch (X-men #4, 1964)
Scott Lang (Avengers #181, 1979)
Shang-Chi (Special Marvel Edition #15, 1973)
Sharon Carter (Tales of Suspense #75, 1966)
She-Hulk (Savage She-Hulk #1, 1980)
SHIELD (Strange Tales #135, 1965)
Shiklah (Deadpool vol. 4 #27, 2014)
Shuri (Black Panther vol. 4 #2, 2005)
Sif (Journey Into Mystery #102, 1964)
Silk (Amazing Spider-Man #4, 2014)
Silver Sable (Amazing Spider-Man #265, 1985)
Silver Surfer (Fantastic Four #48, 1966)
Skaar (What If? Planet Hulk #1, 2007)
Slingshot (Yo-Yo) (Mighty Avengers #13, 2008)
SP//dr (Peni Parker) (Edge of Spider-Verse #5, 2014)
Spider-Girl (Amazing Spider-Man #637, 2010)
Spider-Gwen (Edge of the Spiderverse #2, 2014)
Spider-Man (Amazing Fantasy #15, 1962)
Spider-Man Noir (Spider-Man Noir #1, 2009)
Spider-Woman (Marvel Spotlight #32, 1977)
Spider-Woman II (Marvel Super Heroes: Secret Wars, #7, 1984)
Star Lord (Marvel Preview #4, 1976)
Stinger (Cassie Lang) (The Astonishing Ant-Man #6, 2016)
Storm (Giant-Size X-Men #1, 1975)
Squirrel Girl (Marvel Super Heroes #8 Winter Special, 1992)
Sub-Mariner (Motion Picture Funnies Weekly #1, 1939 / Marvel Comics #1, 1939)
S.W.O.R.D. (Astonishing X-Men #3/#6, 2004)
Thor (Journey into Mystery #83, 1962)
Thor (Jane Foster) (Thor vol. 4 #1, 2014)
Thunderbolts (Incredible Hulk #449, 1997)
Time Variance Authority (Might Thor #372, 1986)
U.S. Agent (Captain America vol. 1 #354, 1989)
Vision, The (Avengers #57, 1968)
Warlock (Fantastic Four #67, 1967)
Wasp (Tales to Astonish #44, 1963)
Wasp (Nadia Van Dyne) (Free Comic Book Day Civil War II, 2016)
Watcher, The (Fantastic Four #13, 1966)
Werewolf by Night (Marvel Spotlight #2, 1972)
Wolverine (Incredible Hulk #180/#181, 1974)
X-Force (The New Mutants #100)
X-Men (X-Men #1, 1963)
X-Statix (X-Force #116, 2001)
X-23 (Nyx #3, 2004)
X-51 (Machine Man) (2001: A Space Odyssey #8, 1977)
Yelena Belova (Inhumans vol. 2 #5, 1999)
Young Avengers (Young Avengers #1, 2005)
Marvel Villains First Appearances #
Abomination (Tales to Astonish #90, 1967)
Apocalypse (X-Factor #5/6)
Baron Zemo (Captain America #164, 1973)
Batroc the Leaper (Tales of Suspense #75, 1966)
Black Cat (Amazing Spider-Man #194, 1979)
Blackheart (Daredevil vol. 1 #270, 1989)
Blob, The (X-Men #3, 1964)
Boba Fett (Star Wars #42, 1980)
Bullseye (Daredevil #131, 1975)
Carnage (Amazing Spider-Man #361, 1992)
Cletus Kasady (Amazing Spider-Man #344/345, 1991)
Crossbones (Captain America #360, 1989)
Cyttorak the Destroyer (Doctor Strange: Sorcerer Supreme #44, 1992)
Daken (Wolverine Origins #10, 2007)
Dark Avengers (Dark Avengers #1, 2009)
Dark Scarlet Witch (Wanda Maximoff) (Avengers West Coast #56, 1990)
Dark Phoenix (X-Men #134, 1980)
Death (Captain Marvel #27, 1973)
Doctor Doom (Fantastic Four #5, 1962)
Doctor Octopus (Amazing Spider-Man #3, 1963)
Dormammu (Strange Tales #126–127, 1964)
Eddie Brock (Web of Spider-Man #18, 1986)
Electro (Amazing Spider-Man #9)
Emma Frost (X-Men #129, 1980)
Evil Deadpool (Deadpool vol. 4 #45, 2011)
Fin Fang Foom (Strange Tales #89, 1961)
Galactus (Fantastic Four #48, 1966)
Gorr the God Butcher (Thor: God of Thunder #2, 2013)
Green Goblin (Amazing Spider-Man #14)
Green Goblin II (Harry Osborn) (Amazing Spider-Man vol. 2 #136, 1974)
High Evolutionary (The Mighty Thor vol. 1 #134, 1966)
Hobgoblin (Amazing Spider-Man #238, 1983)
Hood, The (The Hood #1, 2001)
Iron Patriot (Norman Osborn) (Dark Avengers #1, 2009)
Juggernaut (X-Men #12, 1965)
Kang (Avengers #8, 1964)
Kingpin (Amazing Spider-Man #50, 1967)
Knull, God of the Symbiotes (unnamed) (Thor: God of Thunder #6, 2013)
Knull (God of the Symbiotes) (Venom vol. 4 #3, 2018)
Kraven (Amazing Spider-Man #15, 1964)
Kree (Fantastic Four #65, 1967)
Lady Bullseye (Daredevil vol. 2 #111, 2008)
Lady Taskmaster (Deadpool Max #5, 2011)
Lizard (Amazing Spider-Man #6, 1963)
Loki (Journey Into Mystery #85, 1962)
Madame Hydra (Valentina Allegra de la Fontaine) (Secret Warriors #2, 2009)
Maestro (The Incredible Hulk: Future Imperfect #1, 1992)
Malekith (The Mighty Thor #344, 1984)
Mandarin, The (Tales of Suspense #50, 1964)
Man Wolf (Amazing Spider-Man vol. 1 #124, 1973)
Marvel Zombies (Ultimate Fantastic Four #21/#22, 2005)
Maximus the Mad (Fantastic Four #47, 1966)
Mephista (Jezebel) (Doctor Strange: Sorcerer Supreme #6, 1989)
Mephisto (Silver Surfer #3, 1968)
Minotaur (Dario Agger) (Thor: God of Thunder #19, 2014)
Mister Negative (Free Comic Book Day: The Amazing Spider-Man #1, 2007)
Mister Sinister (Uncanny X-Men #221, 1987)
MODOK (Tales of Suspense #94, 1967)
Morbius (Amazing Spider-Man #101, 1971)
Morlun (Vlad | Inheritors) (Amazing Spider-Man vol. 2 #30)
Mysterio (Amazing Spider-Man #13, 1964)
Mystique (Ms Marvel #18, 1978)
Necromancer (Stephen Strange) (Doctor Strange: Sorcerer Supreme #46, 1992)
Omega Red (X-Men vol. 2 #4, 1992)
Phoenix, The (X-Men #101, 1976)
Red Skull (Captain America Comics #7, 1941)
Rhino, The (Amazing Spider-Man #41, 1966)
Sabretooth (Iron Fist #14, 1977)
Sandman (Amazing Spider-Man #4, 1963)
Scream (Donna Diego) (Venom: Lethal Protector #4, 1993)
Scorn (Tanis Nieves) (Carnage #1, 2010)
Scorpion, The (Amazing Spider-Man #20, 1965)
Sentinels (X-Men #14, 1965)
Shocker, The (Amazing Spider-Man #46, 1967)
Shuma Gorath (Marvel Premiere #10, 1973)
Sinister Six (Amazing Spider-Man Annual #1, 1964)
Skrulls (Fantastic Four #2, 1962)
Starling (Tiana Toomes) (Miles Morales: Spider-Man #6, 2019)
Tarantula (Amazing Spider-Man vol. 1 #134, 1974)
Taskmaster (Avengers #195/196, 1980)
Thane (Son of Thanos) (New Avengers vol. 3 #10, 2013)
Thanos (Iron Man #55, 1973)
Thundra (Fantastic Four vol. 1 #129, 1972)
Tombstone (Web of Spider-Man #36, 1988)
Typhoid Mary (Daredevil #254, 1988)
Ultron (Avengers #54/55, 1968)
Venom Symbiote (Marvel Super Heroes: Secret Wars #8, 1984)
Venom (Amazing Spider-Man #299/300, 1988)
Venom II (Mac Gargan) (Marvel Knights: Spider-Man #10, 2005)
Venom III (Flash Thompson) (Amazing Spider-Man vol. 1 #654, 2011)
Vulture (Amazing Spider-Man #2, 1963)
Weapon H (The Totally Awesome Hulk #22, 2017)
Weapon X Program (Marvel Comics Presents #72, 1991)
Wendigo (Incredible Hulk vol. 1 #162, 1973)
Winter Soldier (Captain America vol. 5 #6, 2005)
Marvel – Other #
Amazing Adventures #11 (1972) — Beast now Furry
Amazing Spider-Man #121 (1973) — Death of Gwen Stacy
Amazing Spider-Man #122 (1973) — Death of Green Goblin
Amazing Spider-Man #400 (1995) — Death of Aunt May (Imposter)
Avengers #4 (1964) — First Silver Age Captain America
Captain America vol. 5 #25 (2007) — Death of Captain America
Daredevil #7 (1965) — First appearance of Red Costume
Daredevil #181 (1982) – Death of Electra
Hulk #2 (1962) — First Green Hulk
Incredible Hulk #340 (1988) — Iconic Cover
Incredible Hulk Special #1 (1968) — Iconic Cover
Marvel Premiere #47 (1979) — First Scott Lang as Ant-Man
Sgt. Fury and His Howling Commandos #13 (1964) — Nick Fury meets Captain America
Silver Surfer #44 (1990) — First appearance of Infinity Gauntlet
Star Wars #1 (1977) — Key Issue
Star Wars #107 (1986) — Scare last issue
Strange Tales #114 (1963) — First Silver Age “Captain America” (Imposter)
Strange Tales #169 (1973) — First solo Doctor Strange title
Thor: God of Thunder #2 (2013) — First appearance of All-Black the Necrosword
Uncanny X-Men #142 (1981) — Days of Future Past Part Two
Web of Spider-Man #118 (1994) — Modern Key Issue
X-Men #141 (1981) — Days of Future Past Part One
DC Heroes First Appearances #
Amanda Waller (DC Legends #1, 1986)
Aquagirl (Adventure Comics #266, 1959)
Aquaman (More Fun Comics #73, 1941)
Atom, The (All-American Comics #19, 1940)
Barry Allen (Showcase #4, 1956)
Batgirl (Batman #139, 1961)
Batgirl II (Cassandra Cain) (Batman #567, 1999)
Batman (Detective Comics #27, 1939)
Batman III (Damian Wayne) (Batman #666, 2007)
Batwoman (Detective Comics #233, 1956)
Batwoman III (52 #11, 2006)
Big Barda (Mister Miracle #4, 1971)
Black Canary (Flash Comics #86, 1947)
Bluebird (Harper Row) (Batman vol. 2 #28, 2014)
Cyborg (DC Comics Presents #26, 1980)
Constantine (Saga of the Swamp Thing #37, 1985)
Damian Wayne (Batman #655, 2006)
Deadman (Strange Adventures #205, 1967)
Elongated Man (Flash #112, 1960)
Etrigan the Demon (The Demon #1, 1972)
Firestorm (Firestorm #1, 1978)
Flash, The (Flash Comics #1, 1940)
Green Arrow (More Fun Comics #73, 1941)
Green Lantern (All-American Comics #16, 1940)
Guy Gardner (Green Lantern #59, 1968)
Hal Jordan (Showcase #22, 1959)
Hawk and Dove (Showcase #75, 1968)
He-Man (DC Comics Presents #47, 1982)
Huntress II (DC Super-Stars #17, 1977)
Jason Todd (Batman #357, 1983)
John Stewart (Green Lantern #87, 1972)
Jonah Hex (All-Star Western vol. 2 #10, 1972)
Justice League (Brave and the Bold #28, 1960)
Kamandi (Kamandi, The Last Boy on Earth #1, 1972)
Kyle Rayner (Green Lantern vol. 3 #48, 1994)
Kilowog (Green Lantern #201, 1986)
Martian Manhunter (Detective Comics #225, 1955)
Mera (Aquaman #11, 1963)
Metamorpho, (The Brave and the Bold #57, 1965)
Mister Miracle (Mister Miracle #1, 1971)
Nightwing (Tales of the Teen Titans #44, 1984)
Orion (The New Gods #1, 1971)
Power Girl (All Star Comics #58, 1976)
Raven (DC Comics Presents #26, 1980)
Robin II (Jason Todd) (Batman #366, 1983)
Robin III (Tim Drake) (Batman #442, 1989)
Roy Harper (More Fun Comics #73, 1941)
Sgt. Rock (Our Army At War #83, 1959)
Signal, The (Duke Thomas) (Batman vol. 2 #21, 2013)
Starfire (DC Comics Presents #26, 1980)
Superboy (More Fun Comics #101, 1945)
Supergirl (Action Comics #252, 1959)
Superman (Action Comics #1, 1938)
Spectre, The (More Fun Comics #52, 1940)
Swamp Thing (House of Secrets #92, 1971)
Terra (The New Teen Titans #26, 1982)
Tim Drake (Batman #436, 1989)
Timothy Fox (Jace Fox, Future State New Batman) (Batman #313, 1979)
Unknown Soldier (Our Army at War #168, 1966)
Wally West (The Flash #110, 1959)
Wonder Girl (The Brave and the Bold #60, 1965)
Wonder Twins (Super Friends #7, 1977)
Wonder Woman (All-Star Comics #8, 1941)
Zatanna (Hawkman #4, 1964)
DC Villains First Appearances #
Bane (Bane: Vengeance of Bane #1, 1993)
Batman Who Laughs (Teen Titans #12, 2017)
Bizzaro (Superboy #68, 1958)
Black Adam (Marvel Family #1, 1945)
Black Hand (Green Lantern #29, 1964)
Black Manta (Aquaman #35, 1967)
Black Mask (Batman #386, 1985)
Brainiac (Action Comics #242, 1958)
Catwoman (Batman #1, 1940)
Cheetah (Wonder Woman #6, 1943)
Clayface (Detective Comics #40, 1940)
Court of Owls (Batman vol. 2 #6, 2012)
Darkseid (Superman’s Pal Jimmy Olsen #134, 1970)
Deathstroke (The New Teen Titans #2, 1980)
Doomsday (Superman: The Man of Steel #18, 1992)
Eclipso (House of Secrets #61, 1963)
Gorilla Grodd (Flash #106, 1959)
Harley Quinn (Batman Adventures #12, 1993)
Hunter Zolomon (Flash Secret Files #3, 2001)
Hush (Thomas Elliot) (Batman #609, 2003)
Isis (Shazam #25, 1976)
Joker (Batman #1, 1940)
Joker’s Daughter (Batman Family #6, 1976)
Killer Croc (Detective Comics #523/Batman #357/Detective Comics #524, 1983)
League of Assassins (Detective Comics #405, 1970)
Lex Luthor (Action Comics #43, 1940)
Livewire (Superman Adventures #5, 1997)
Lobo (The Omega Men #3, 1983)
Mister Mxyzptlk (Superman #30, 1944)
Mongul (DC Comics Presents #27, 1978)
Mr. Freeze (Batman #121, 1959)
Ocean Master (Aquaman #29, 1966)
Parasite (Action Comics #340, 1966)
Penguin (Detective Comics #58, 1941)
Poison Ivy (Batman #181, 1966)
Punchline (Batman vol. 2 #89, 2020)
Ra’s Al Ghul (Batman #232, 1971)
Red Hood, The (Detective Comics #158, 1951)
Red Hood II (Jason Todd) (Batman #635, 2005)
Reverse Flash (Eobard Thawne) (The Flash #139, 1963)
Riddler, The (Detective Comics #140, 1948)
Sinestro (Green Lantern #7, 1961)
Solomon Grundy (All-American Comics, 1944)
Talia Al Ghul (Detective Comics #411, 1971)
Two-Face (Detective Comics #66, 1942)
Ventriloquist (Detective Comics #583, 1988)
Victor Zsasz (Batman: Shadow of the Bat #1, 1992)
White Rabbit (Jaina Hudson) (Batman: Dark Knight vol. 2 #3, 2012)
DC – Other #
Batman #171 (1965) — First Silver Age Riddler
Batman #423 (1988) — Modern Key Issue
Batman #428 (1989) — Death of Robin II (Jason Todd)
Batman #497 (1993) — Bane breaks Batman’s back
Detective Comics #298 (1961) — First Silver Age Clayface
Detective Comics #359 (1967) — First Barbra Gordon as Batgirl
Detective Comics #880 (2011) — Iconic Cover
Flash, The #323, 324, 325 (1983) — Death of Professor Zoom
Green Lantern #76 (1970) — Iconic Cover
Green Lantern #85 (1971) — Heroine Abuse Issue
Shazam #28 (1977) — First appearance of Black Adam since Golden Age
Wonder Woman #98 (1958) — First Silver Age Wonder Woman
Non-Marvel/DC First Appearances #
Admiral Thrawn (Star Wars: Heir to the Empire #1, 1995)
Archie Andrews (Pep Comics #22, 1941)
Betty Cooper (Pep Comics #22, 1941)
Cheryl Blossom (Archie’s Girls: Betty and Veronica, 1982)
Groo the Wanderer (Destroyer Duck #1, 1982)
Hellboy (San Diego Comic-Con Comics #2, 1993)
Josie McCoy (Archie’s Pals’n’Gals #23, 1963)
Jughead (Pep Comics #22, 1941)
Little Lulu (The Saturday Evening Post, 23 February 1935)
Sabrina the Teenage Witch (Archie’s Madhouse #22, 1962)
Sara Pezzini (Witchblade) (Cyblade / Shi: Battle for Independents #1, 1995)
Tick, The (New England Comics Newsletter #14, 1986)
Usagi Yojimbo (Albedo Anthropomorphics #2, 1984)
Vampirella (Vampirella #1, 1969)
Veronica Lodge (Pep Comics #22, 1941)
Non-Marvel/DC Key Issues: #
Bone #1 (1991) — Modern Key Issue
Dime Press #4 (1993) — Hellboy Appearance on Cover Predating First U.S. Appearance
Four Color Comics #16 (1941) — First Mickey Mouse in comics
Gobbledygook #1 (1984) — Rare Publication by Eastman and Laird
Gobbledygook #2 (1984) — Rare Publication by Eastman and Laird
Goon #1 (1999) — First Appearance and Low Distribution
Goon #2 (1999) — Low Distribution
Goon #3 (1999) — Low Distribution
Hellblazer #1 (1988) — Modern Key Issue and First Solo Constantine Title
Invincible #1 (2003) — Modern Key Issue
John Bryne’s Next Men #21 (1993) — First Full Color Hellboy Appearance
Phantom #1 (1962) — First Gold Key Appearance of The Phantom
Preacher #1 (1995) — Modern Key Issue
Rudolph the Red-Nosed Reindeer (1939) — First Rudolph in any medium
Sandman #1 (1989) — Modern Key Issue
Spawn #119 (2002) — Modern Key Issue, Low print run
Spawn #122 (2003) — Modern Key Issue, Low print run
Spawn #139 (2004) — Modern Key Issue, Low print run
Spawn #141 (2004) — Modern Key Issue, Low print run
Spawn #165 (2007) — Modern Key Issue, Low print run
Spawn #174 (2008) — Modern Key Issue, Low print run
Spawn #175 (2008) — Modern Key Issue, Low print run
Spawn #183 (2008) — Modern Key Issue, Low print run
Spawn #216 (2012) — Modern Key Issue, Low print run
Spawn #221 (2012) — Modern Key Issue, Homage Cover
Spawn #227 (2013) — Modern Key Issue, Homage Cover
Spawn #228 (2013) — Modern Key Issue, Homage Cover
Spawn #230 (2013) — Modern Key Issue, Homage Cover
Spawn #231 (2013) — Modern Key Issue, Homage Cover
Spawn #240 (2014) — Modern Key Issue, Low print run
Spawn #241 (2014) — Modern Key Issue, Low print run
Spawn #243 (2014) — Modern Key Issue, Low print run
Tank Girl #1 (1990) — Modern Key Issue
The Walking Dead #1 (2003) — Modern Key Issue
Y: The Last Man #1 (2002) — Modern Key Issue